Oral presentation
alternative RNA splicing, pre-mRNA splicing, spliceosomes, CRISPR, high-throughput assay
Public Summary
We scaled haploid CRISPR-Cas9 base editing to target the human spliceosome and interrogated the mutants using the U2 snRNP/SF3b inhibitor, pladienolide B. Our approach provides a template for the analysis of essential cellular machines in humans.
Presenter
Irene Beusch, PhD
Postdoc
Hiten Madhani, University of California, San Francisco
Other Attending Contributors
Em SeGraves
Undergraduate Student
Hiten Madhani, University of California, San Francisco
Hiten Madhani, MD, PhD
Lab Head
Hiten Madhani, University of California, San Francisco