Nanopore full-length mRNA sequencing supports endonucleolytic cleavage, but not deadenylation, as a mechanism of Nonsense-Mediated mRNA Decay

Type of Presentation: Poster Presentation
Topics: mRNA decay, enzymes cleaving RNA, RNA polyadenylation, Translation, Nonsense mediated decay, nanopore sequencing

Public Summary

Using nanopore dRNA-seq and C. elegans genetics, we studied metazoan Nonsense-Mediated mRNA Decay (NMD). Our results support that SMG-5/6/7 act together in one endonuclease reaction, and that deadenylation is not a constitutive step of NMD.

Presenter

Marcus Viscardi

Graduate Student

Joshua Arribere, University of California, Santa Cruz

Other Attending Contributors

Joshua Arribere

Lab Head

Joshua Arribere, University of California, Santa Cruz

Nanopore full-length mRNA sequencing supports endonucleolytic cleavage, but not deadenylation, as a mechanism of Nonsense-Mediated mRNA Decay

Public Summary

Presenter

Marcus Viscardi

Other Attending Contributors

Joshua Arribere

Related Presentations

Principles and properties of disordered regions in post-transcriptional control

Exploring a novel requirement for a PARK9 homolog in No-Go mRNA Decay in C. elegans

3:00pm - 3:10pm

Ubiquitination of stalled ribosomes enables mRNA decay via HBS-1 and NONU-1 in vivo

Questions?

About The Bay Area RNA Club