Said Goueli, PhD

Lab Head

Said Goueli, Promega Corp.

Pronouns: he/him

Said A. Goueli, Ph.D.
Senior Research Fellow, and Group Leader of Cell Signaling
Promega Corporation
Said Goueli is a Senior Research Fellow at Promega Corporation. He is the founder of the cell signaling group, where his focus is mainly directed at biochemical assays to study cell signaling and, most recently, on cell based technologies to monitor cellular metabolites in normal and abnormal cell growth. In addition to his role at Promega. Said is a Clinical Professor at the University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
Said has pioneered novel technologies that advanced research in cell signaling. He was one of the first to develop anti phosphospecific antibodies, and holds over 15 issued and several pending patents on kinase assays (radioactive, fluorescent, and luminescent) and related technologies. He has also developed novel technologies in monitoring the activity of protein phosphatases and is currently developing luminescent assays for monitoring the activity of phospholipid phosphatases.
Said has also developed a novel strategy to monitor the modulation of Gs and Gi-Protein Coupled Receptors (GPCR) and Phosphodiesterases. His interest has expanded to newer areas of cell signaling research as evidenced by his development of assays to address the need of research in the epigenome. His most recent development is a universal homogenous bioluminescent assay for all classes of methyltransferases.
Said most recent activities are devoted to monitoring and detection of the activity of small GTPases such as RAS, RHO, RAK, CDC42, etc. which are intimately involved in the development and proliferation of almost all kinds of tumors and play pivotal role in tumor invasiveness and metastasis.
The quality of air, food, and water has recently draw Said attention to develop novel approaches to detect bacteria, yeast, and spores and thus can be used as quality control measures.
Prior to joining Promega, Said was at the University of Minnesota, where he made seminal contributions to the molecular action of androgens on modulating gene expression in the prostate.
Said has published over 100 peer reviewed journals and over 15 chapters, as well as organized many symposia on cell signaling. He also is a Co- editor of a volume on Protein Kinases in the series of “Methods in Molecular Biology”. He has over 16 Patents in the areas of kinases and GPCR. He holds a Bachelor of Science degree and Master of Science degree in Biochemistry from the University of Alexandria, Egypt, a Ph.D. from the University of Maine, and postdoctoral training at the University of Toledo School of Medicine on the role of PKA in cellular regulation.
Contact:
Email – [email protected]
Phone – 608-277-2650
Selected Publications:

1. Kevin Hsiao, Hicham Zegzouti, Said Goueli (2022). High throughput Bioluminescent assay to characterize and monitor the activity of SARS-CoV-2 Methyltransferases (2022). COVID-19 SARS-CoV-2 preprints from medRxiv and bioRxiv. doi: https://doi.org/10.1101/2022.09.12.505486
2. Kevin Hsiao, Hicham Zegzouti, Said Goueli (2022). High throughput bioluminescent assay to characterize and monitor the activity of SARS-CoV-2 methyltransferases. PLOS ONE Published: November 29, 2022. https://doi.org/10.1371/journal.pone.0274343
3. Subhanjan Mondal, Nathan Feirer, Michael Brockman, Melanie A. Preston, Sarah J. Teter, Dongping Ma, Said A. Goueli, Sameer Moorji, Brigitta Saul, James J. Cali (2021). A Direct Capture Method for Purification and Detection of Viral Nucleic Acid Enables Epidemiological Surveillance of SARS-CoV-2.doi: https://doi.org/10.1101/2021.05.06.21256753
4. Subhanjan Mondal, Nathan Feirer, Michael Brockman, Melanie A. Preston, Sarah J. Teter, Dongping Ma, Said A. Goueli, Sameer Moorji, Brigitta Saul, James J. Cali (2021) A direct capture method for purification and detection of viral nucleic acid enables epidemiological surveillance of SARS-CoV-2. Science of The Total Environment Volume 795, 15 November 2021, 148834. https://doi.org/10.1016/j.scitotenv.2021.148834
5. Laurie Engel ,Juliano Alves, Jacquelyn Hennek, Said A. Goueli, and Hicham Zegzouti (2021). Utility of Bioluminescent Homogeneous Nucleotide Detection Assays in Measuring Activities of Nucleotide-Sugar Dependent Glycosyltransferases and Studying Their Inhibitors. Molecules 2021, 26(20), 6230; https://doi.org/10.3390/molecules26206230
6. Matthew Swiatnicki, Laurie Engel, Riva Shrestha, Juliano Alves, Said A Goueli, Hicham Zegzouti (2022) Profiling oncogenic KRAS mutant drugs with a cell-based Lumit p-ERK immunoassay. SLAS Discov. Mar 11; S2472-5552(22)12517-7. doi: 10.1016/j.slasd.2022.03.001. Online ahead of print
7. Juliano Alves, Laurie Engel, Renata de Vasconcelos Cabral, Eduardo L Rodrigues, Liane de Jesus Ribeiro, Luiza M Higa, Orlando da Costa Ferreira Júnior, Terezinha Marta P P Castiñeiras, Isabela de Carvalho Leitão, Amilcar Tanuri, Said A Goueli, Hicham Zegzouti (2021) A bioluminescent and homogeneous SARS-CoV-2 spike RBD and hACE2 interaction assay for antiviral screening and monitoring patient neutralizing antibody levels. Sci Reports. 11(1):18428. doi: 10.1038/s41598-021-97330-3
8. Laurie Engel, Juliano Alves, Jacquelyn Hennek, Said A. Goueli, and Hicham Zegzouti (2021) Utility of Bioluminescent Homogeneous Nucleotide Detection Assays in Measuring Activities of Nucleotide-Sugar Dependent Glycosyltransferases and Studying Their Inhibitors Molecules, 26(20), 6230; https://doi.org/10.3390/molecules26206230
9. Subhanjan Mondal, Nathan Feirer, Michael Brockman, Melanie A. Preston, Sarah J. Teter, Dongping Ma, Said A. Goueli, Sameer Moorji, Brigitta Saul, James J. Cali (2021). MedRxiv: https://doi.org/10.1101/2021.05.06.21256753. A Direct Capture Method for Purification and Detection of Viral Nucleic Acid Enables Epidemiological Surveillance of SARS-CoV-2
10. Abushaban, A; Salinas-Rodriguez, SG; Goueli, S; Mondal, M; Schippers, JC; and Kennedy, M (2020). Monitoring Biofouling Potential Using ATP-Based Bacterial Growth Potential in SWRO Pre-Treatment of a Full-Scale Plant. Membranes, volume 10: Issue 11, 360
11. Huang, B, Engel, L, Goueli, SA, and Zegzouti, H (2020) A homogeneous bioluminescent immunoassay to probe cellular signaling pathway regulation. Nature Communication Biology, 3: 8. Published online 2020 Jan 3. doi: 10.1038/s42003-019-0723-9
12. Abushaban, A, Salinas-Rodriguez, SG, Kapala, M, Pastorelli, D, Schippers, JC, Mondal, S, Goueli, SA, and Kennedy, MD (2020) Correlating bacterial growth potential measurements with fouling development in a full-scale SWRO system. Desalination (2020) In Review
13. Goueli, SA and Hsiao, K (2019) Monitoring and characterizing soluble and membrane-bound ectonucleotidases CD73 and CD39.
PLoS One. 2019 Oct 25;14(10): e0220094.
doi: 10.1371/journal.pone.0220094. eCollection 2019
14. Goueli, SA and Kevin Hsiao (2019) Monitoring and characterizing soluble and membrane-bound ectonucleotidases CD73 and CD39
bioRxiv 698373; doi: https://doi.org/10.1101/698373
15. Abushaban, A, Salinas-Rodriguez, SG, Nasir Mangal, M, Mondal, S, Goueli, SA, Knezev, A, Vrouwenvelder, JS, Schippers, JC, and Kennedy, MD. ATP measurement in seawater reverse osmosis systems: Eliminating seawater matrix effects using a filtration-based method. Desalination; 453, 2019, 1-9
16. Abushaban, A, Salinas-Rodriguez, SG, Mondal, S, Goueli, SA, and Kennedy, MD. (2017). A new method of assessing bacterial growth in seawater reverse osmosis systems: method development and applications (2017). The International Desalination Association World Congress, São Paulo, Brazil; Volume: IDA17WC-58031
17. Abushaban, ¬¬A, Mangal, MN, Salinas-Rodriguez SG, Nnebuo, C, Mondal, S, Goueli, SA, Schippers, JC, and Kennedy, MD (2017) Direct measurement of ATP in seawater and application of ATP to monitor bacterial growth potential in SWRO pre-treatment systems. Desalination and Water Treatment, 99:91-101
18. Alves J, Vidugiris G, Goueli SA, Zegzouti H (2017) Bioluminescent High-Throughput Succinate Detection Method for Monitoring the Activity of JMJC Histone Demethylases and Fe(II)/2-Oxoglutarate-Dependent Dioxygenases. SLAS Discov. 2017 ec 1:2472555217745657. doi: 10.1177/2472555217745657. [Epub ahead of print]
19. Mondal S, Hsiao K, Goueli SA (2017) Utility of Adenosine Monophosphate Detection System for Monitoring the Activities of Diverse Enzyme Reactions. Assay Drug Dev Technol...15; 330-341.
20. Hsiao K, Alves J, Patel R, Adams M, Nashine V, Goueli S. (2017) A High-Throughput Bioluminescent Assay to Monitor the Deamidation of Asparagine and Isomerization of Aspartate Residues in Therapeutic Proteins and Antibodies. J Pharm Sci. 106:1528-1537.
21. Worzella T, Butzler M, Hennek J, Hanson S, Simdon L, Goueli S, Cowan C, Zegzouti H.A (2017) Flexible Workflow for Automated Bioluminescent Kinase Selectivity Profiling. SLAS Technol. 22:153-162
22. Goueli, SA (2017) Kinases and Drug Discovery: Protein Kinases as a Validated Drug Target (2107) Drug Discovery World. pp53-60
23. Zegzouti, H and Goueli, SA. Editors (2016): Methods in Molecular Biology: Kinase Screening and Profiling; Methods and Protocols. Humana Press.
24. Zegzouti, H, Hennek, J, and Goueli, SA (2016) Using Bioluminescent Kinase Profiling Strips to Identify Kinase Inhibitor Selectivity and Promiscuity. In “Methods in Molecular Biology: Kinase Screening and Profiling; Methods and Protocols; (Zegzouti and Goueli, eds.) Humana Press”. pp 59-73.
25. Hennek J, Alves J, Yao E, Goueli SA, and Zegzouti H (2016) Bioluminescent kinase strips: A novel approach to targeted and flexible kinase inhibitor profiling. Anal. Biochem. 495, 9-20
26. Mondal S, Hsiao, K, and Goueli, SA (2016) A bioluminescent assay for monitoring conjugation of ubiquitin and ubiquitin-like proteins. Anal. Biochem. 510, 41-51
27. Hsiao, K; Zegzouti, H; and Goueli, SA (2016) Methyltransferase-Glo: A universal, bioluminescent and homogenous assay for monitoring all classes of methyltransferases. J. Epigenomics, Vol. 8, 321-339.
28. Mondal S, Hsiao K, Goueli SA (2015) A Homogenous Bioluminescent System for Measuring GTPase, GTPase Activating Protein, and Guanine Nucleotide Exchange Factor Activities. Assay Drug Dev Technol. 8:444-455
29. Goueli, SA, Zegzouti, H, and Hsiao K (2014) AMP Glo, Luminescent assay for diverse Biochemical Reactions. GEN, August issue
30. Zegzouti, H; Zdanovskaia, M; Hsiao, K; and Goueli, SA (2009) ADP-GLO: A Bioluminescent and homogenous ADP monitoring assay for kinases. Assay Drug Dev. Technol. 7: 560-572.
31. Larson, B; Banks, P; Zegzouti, H; and Goueli, SA (2009) A simple and robust automated kinase profiling platform using luminescent ADP accumulation technology. Assay Drug Dev. Technol. 7: 573-584
32. Vidugiriene, J; Zegzouti, H; and Goueli, SA (2009) Evaluating the utility of a bioluminescent ADP-detecting assay for lipid kinases. Assay Drug Dev. Technol. 7: 585-597
33. El Awady, MK; Azzazy, HM; Fahmy, AM; Shawky, SM; Badreldin, NG, Yossef, SS; Omran, MH, Zekri, AR, and Goueli, SA (2009) Positional effect of mutations in 5’UTR of hepatitis C virus 4a on patients’ response to therapy. World J Gastroenterology 15: 1480-1486
34. Shultz, S; Worzella, T; Gallagher, A; Shieh, J; Goueli, SA, Hsiao, K; and Vidugiriene, J (2008) Miniaturized GPCR signaling studies in 1536-well format. J. Biomed Technol. 19: 267-274
35. Kumar, M; Hsiao, K; Vidugiriene, J; and Goueli, SA (2007) A bioluminescent-based HTS-compatible assay to monitor G-protein coupled receptor modulation of cellular cyclic AMP. Assay Drug Dev. Technol. 5: 237-245
36. El-Awady, MK; Tabll, AA; El-Abd, YS; Bahgat, MM; Shoeb, HA; Youssef,SS; Bader El Din, NG; Redwan, EM; Omran, MH; El-Demellawy, M; El-Garf , WT; and Goueli, SA (2006) HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro. World J Gastroenterol 12: 4836-4842
37. El Awady, MK; Badr El Din, NG; El Garf, WT;Youssef, SS;Omran, MH; El Abd, J; and Goueli, SA (2006) Antisense oligonucleotide inhibition of hepatitis C virus genotype 4 replication in HepG2 cells. Cancer Cell International 6; 18
38. Goueli, S. A; Hsiao, K; and Bulleit, B (2006) A Homogenous, Luminescent, High-Throughput, Versatile Assay for a Wide Range of Kinases In “Handbook of Assay Development in Drug Discovery” Lisa Minor, Editor Chapter 8, pp 99-114. Taylor & Francis Group, LLC. CRC, Boca Raton, FL
39. Kupcho, K; Hsiao, K; Bulleit, B, and Goueli, S. A.(2004) A Homogenous, Non-radioactive High-Throughput Fluorogenic Protein Phosphatase Assay. Journal of Biomol. Screening, 9(3)223-231.
40. Kupcho, K; Somberg1, R; Bulleit, B, and Goueli, S.A (2003) A Homogenous, Non-radioactive High Throughput Fluorogenic Protein Kinase Assay. Anal. Biochem., 317, 210-217
41. Kelemen, B.R., Hsiao, K, and Goueli, S.A. (2002) Selective in vivo inhibition of mitogen-activated protein kinase activation using cell-permeable peptides. J. Biol. Chem., 277, 8741-8748.
42. El Awady, M.K, El-Demellawy, M.A., Khalil, S.B., Galal, D., and Goueli, S.A (2002) Synthetic peptide-based immunoassay as a supplemental test for HCV infection. Clin Chim Acta, 325 (1-2), 39-47.
43. Curtin M, Watson J, Goueli S (2002) From one well to 9000: using high-density streptavidin-coated membranes for kinase detection. Biotechniques.Suppl:107-11.
44. Goueli, S.A. and Jarvis, B (2001) Phosphospecific MAPK antibodies for ERK, JNK, and p38 activation. In”Methods in Enzymology: Ras regulators and effectors”, Vol 332, 337-343, Der, CJ, Hall, A, and Balch, WE, eds. Academic Press, NY.